产品货号：
GS2221
中文名称：
PspCI内切酶
英文名称：
PspCI
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
相关搜索：PspCI内切酶，PspCI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CAC↑GTG |
| 来源 | Pseudomonas species C |
| Assayed on | Lambda DNA |
| Optimal buffer | B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+?BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;10mM2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol.|Storage temperature:-20℃.|For long term storage(more than 30 days),store at -70oC. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.Ligation is better in presence of 10%PEG. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AcvI,BbrPI,Eco72I,PmaCI,PmlI |
相关搜索：PspCI内切酶，PspCI限制性内切酶