产品货号：
GS2220
中文名称：
AjnI内切酶
英文名称：
AjnI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
At 37℃ activity is about 10%from maximum.
技术规格
相关搜索：AjnI内切酶，AjnI限制性内切酶
使用说明
At 37℃ activity is about 10%from maximum.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | ↑CCWGG |
| 来源 | Acinetobacter johnsonii R2 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT. |
| Optimal temperature | 55℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 6 u.a.of enzyme for 16hoursat 55℃. |
| Methlation effect | Not blocked by dcm methylation:CCWGG |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BciT130I^,BptI^,BseBI^,BsiLI^,Bst2UI^,BstNI^,BstOI^,EcoRII^,MvaI^,Psp6I?^,PspGI^ |
相关搜索：AjnI内切酶，AjnI限制性内切酶