产品货号:
GS2204
中文名称:
BstPAI内切酶
英文名称:
BstPAI
产品规格:
500U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)
使用说明
High enzyme concentration and incubation at 65℃ for 16hoursresults in star activity.
技术规格
相关搜索:BstPAI内切酶,BstPAI限制性内切酶
使用说明
High enzyme concentration and incubation at 65℃ for 16hoursresults in star activity.
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
Recognition sites | GACNN↑NNGTC |
来源 | Bacillus stearothermophillus PA |
Assayed on | Lambda DNA |
Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
Optimal temperature | 65℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;200 μg/ml BSA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃. |
Ligations | After 5-fold overdigestion with enzyme less than 5%of the DNA fragments can be ligated |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 25℃ |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | NO |
Campatible Ends | BoxI,PshAI |
相关搜索:BstPAI内切酶,BstPAI限制性内切酶