产品货号：
GS2202
中文名称：
SmiMI内切酶
英文名称：
SmiMI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：SmiMI内切酶，SmiMI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CAYNN↑NNRTG |
| 来源 | Sphingobacterium mizutae M |
| Assayed on | Lambda DNA |
| Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | MslI,RseI^ |
相关搜索：SmiMI内切酶，SmiMI限制性内切酶