产品货号：
GS2197
中文名称：
Bso31I内切酶
英文名称：
Bso31I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Bso31I内切酶，Bso31I限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | GGTCTC(N)1↑ |
| 来源 | Bacillus stearothermophilus 31 |
| Assayed on | T7 DNA |
| Optimal buffer | O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 55℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 90%of DNA fragments can be ligated.Of these 80%can be recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a.of enzyme for 16hoursat 55℃. |
| Methlation effect | Not blocked by methylation GGTCTmC |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BsaI,BspTNI,Eco31I |
相关搜索：Bso31I内切酶，Bso31I限制性内切酶