产品货号：
GS2194
中文名称：
BtrI内切酶
英文名称：
BtrI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：BtrI内切酶，BtrI限制性内切酶
使用说明
High enzyme concentration may result in star activity.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
| Recognition sites | CAC↑GTC |
| 来源 | Bacillus stearothermophilus SE-U62 |
| Assayed on | Lambda DNA |
| Optimal buffer | O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 60℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50mMKCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol;Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme 80%of DNA fragments can be ligated.Of these 90%can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a.of enzyme for 16hoursat 60℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AjiI?^,BmgBI^ |
相关搜索：BtrI内切酶，BtrI限制性内切酶