产品货号：
GS2193
中文名称：
PciI内切酶
英文名称：
PciI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.
技术规格
相关搜索：PciI内切酶，PciI限制性内切酶
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | A↑CATGT |
| 来源 | E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45 |
| Assayed on | T7 DNA |
| Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof T7 DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Not blocked by ACmATGT methylation.|Blocked by?mACATGT methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BspLU11I,PscI |
相关搜索：PciI内切酶，PciI限制性内切酶