产品货号：
GS2173
中文名称：
AsuHPI内切酶
英文名称：
AsuHPI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
Enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
技术规格
相关搜索：AsuHPI内切酶，AsuHPI限制性内切酶
使用说明
Enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GGTGA(N)8↑ |
| 来源 | Actinobacillus suis HP |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme about 30%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping dam-methylation(GmATC):GGTGATC |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | HphI |
相关搜索：AsuHPI内切酶，AsuHPI限制性内切酶