产品货号：
GS2163
中文名称：
Bsc4I内切酶
英文名称：
Bsc4I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Bsc4I内切酶，Bsc4I限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | CCNNNNN↑NNGG |
| 来源 | Bacillus schlegelii 4 |
| Assayed on | Lambda DNA |
| Optimal buffer | W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 55℃ |
| Storage conditions | 0 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 30 u.a.of enzyme for 16hoursat 55℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AfiI,BseLI,BsiYI,BslI |
相关搜索：Bsc4I内切酶，Bsc4I限制性内切酶