产品货号：
GS2146
中文名称：
Bsp13I内切酶
英文名称：
Bsp13I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Bsp13I内切酶，Bsp13I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 50℃ in a total reaction volume of 50μl. |
| Recognition sites | T↑CCGGA |
| 来源 | Bacillus species 13 |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | 2K(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;200 mM KCl;1 mM DTT.) |
| Optimal temperature | 50℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 50℃. |
| Methlation effect | Blocked by overlapping Dam methylation(GmATC):TCCGGATC?and?GATCCGGA. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AccIII,Aor13HI,BlfI,BseAI,BsiMI,BspEI,BspMII,Kpn2I,MroI |
相关搜索：Bsp13I内切酶，Bsp13I限制性内切酶