产品货号：
GS2128
中文名称：
VspI内切酶
英文名称：
VspI
产品规格：
1kU
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：VspI内切酶，VspI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | AT↑TAAT |
| 来源 | E.coli strain,that carries the cloned gene VspI from Vibrio species 343 |
| Assayed on | Lambda DNA |
| Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme 70%of the DNA fragments can be ligated.Of these,90%can be recut.In the presence of 10%PEG ligation is better. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃ |
| Methlation effect | Blocked by ATTAmAT methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AseI,AsnI,PshBI |
相关搜索：VspI内切酶，VspI限制性内切酶