产品货号：
GS2119
中文名称：
BstMBI内切酶
英文名称：
BstMBI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：BstMBI内切酶，BstMBI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | ↑GATC |
| 来源 | Bacillus stearothermophillus MB |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50mMKCl;0.1 mM EDTA;200 μg/ml BSA.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃ |
| Methlation effect | Blocked by overlapping Dam methylation(GmATC):?GATC?.|Not blocked by CG methylation.|Not cut hemimethylated site:5- G(6mA)TC-3/3-CTAG-5 |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BfuCI,BscFI,Bsp143I,BssMI,BstENII,BstKTI^,DpnI^,Kzo9I,MalI^,MboI,NdeII^,Sau3AI |
相关搜索：BstMBI内切酶，BstMBI限制性内切酶