产品货号:
GS2084
中文名称:
PctI内切酶
英文名称:
PctI
产品规格:
250U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)
技术规格
相关搜索:PctI内切酶,PctI限制性内切酶
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
Recognition sites | GAATGCN↑ |
来源 | Planococcus citreus SM |
Assayed on | Lambda DNA |
Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
Optimal temperature | 37℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;and 50%glycerol.Store at -20℃. |
Ligations | After 20-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃ |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
Campatible Ends | BsaMI,BscCI,BsmI,Mva1269I,Nb.BsmI^ |
相关搜索:PctI内切酶,PctI限制性内切酶