产品货号：
GS2074
中文名称：
BglI内切酶
英文名称：
BglI
产品规格：
1kU
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity
技术规格
相关搜索：BglI内切酶，BglI限制性内切酶
使用说明
High enzyme concentration may result in star activity
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GCCNNNN↑NGGC |
| 来源 | Bacillus globigii |
| Assayed on | Lambda DNA |
| Optimal buffer | 2W(20mMTris-HCl(pH 8,5 at 25℃);10 mM MgCl2;200 mM NaCl;1 mM DTT) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Not blocked by dcm methylation(CmCWGG):GCCWGGNNGGC |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
相关搜索：BglI内切酶，BglI限制性内切酶