产品货号：
GS2067
中文名称：
AclI内切酶
英文名称：
Acl I
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻(-20℃)
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/mL.Do not use BSA for long incubation.
技术规格
相关搜索：AclI内切酶，AclI限制性内切酶，Acl I
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/mL.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μL. |
| Recognition sites | AA↑CGTT |
| 来源 | Acinetobacter calcoaceticus |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH7.9 at 25℃);10mM magnesium acetate;66mM potassium acetate;1mM DTT.)+ BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mM Tris-HCl(pH7.5);100mM NaCl;0.1mM EDTA;7mM 2-mercaptoethanol;200μg/mL BSA;0.05%Triton X-100;50%glycerol;Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 2 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by CG methylation |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | Psp1406I |
相关搜索：AclI内切酶，AclI限制性内切酶，Acl I