产品货号：
Q10293
中文名称：
重组人颗粒酶B
英文名称：
Recombinant Human Granzyme B Protein (His Tag)
产品规格：
10μg|20μg
发货周期：
1～3天
产品价格：
询价
纯度：>97% as determined by SDS-PAGE
内毒素：<1.0 EU per μg of the protein as determined by the LAL method
表达宿主：HEK293 Cells
种属：Human
预测N端：Gly 19
生物活性：
Measured by its ability to cleave a peptide substrate,tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB).The specific activity is>1500pmol/min/μg.
蛋白构建：
A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.
分子量：
The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27kDa.As a result of glycosylation,the apparent molecular mass of rhGranzyme B is approximately 36kDa in SDS-PAGE under reducing conditions.
缓冲液：
Lyophilized from sterile PBS,pH7.4
Please contact us for any concerns or special requirements.
Normally 5% - 8% trehalose,mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式：
In general,recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid.They are shipped out with blue ice unless customers require otherwise.
稳定性&储存条件：
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃.It is recommended that the protein be aliquoted for optimal storage.Avoid repeated freeze-thaw cycles.
复溶：
A hardcopy of COA with reconstitution instruction is sent along with the products.Please refer to it for detailed information.
背景资料：
Granzyme B,also known as GZMB,is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells.Granzyme B enters the target cells depending on another membrane-binding granule protein,perforin,results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways,and consequently induces rapid cell apoptosis.Over 3 substrates of GZMB have been identified including the key substrate caspase-3,ICAD and Bid.GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells,and accordingly plays an essential role in immunosurveillance.
相关搜索：重组人颗粒酶B，重组人GZMB，人GZMB重组蛋白，Recombinant Human Granzyme B Protein (His Tag)
内毒素：<1.0 EU per μg of the protein as determined by the LAL method
表达宿主：HEK293 Cells
种属：Human
预测N端：Gly 19
生物活性：
Measured by its ability to cleave a peptide substrate,tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB).The specific activity is>1500pmol/min/μg.
蛋白构建：
A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.
分子量：
The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27kDa.As a result of glycosylation,the apparent molecular mass of rhGranzyme B is approximately 36kDa in SDS-PAGE under reducing conditions.
缓冲液：
Lyophilized from sterile PBS,pH7.4
Please contact us for any concerns or special requirements.
Normally 5% - 8% trehalose,mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式：
In general,recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid.They are shipped out with blue ice unless customers require otherwise.
稳定性&储存条件：
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃.It is recommended that the protein be aliquoted for optimal storage.Avoid repeated freeze-thaw cycles.
复溶：
A hardcopy of COA with reconstitution instruction is sent along with the products.Please refer to it for detailed information.
背景资料：
Granzyme B,also known as GZMB,is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells.Granzyme B enters the target cells depending on another membrane-binding granule protein,perforin,results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways,and consequently induces rapid cell apoptosis.Over 3 substrates of GZMB have been identified including the key substrate caspase-3,ICAD and Bid.GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells,and accordingly plays an essential role in immunosurveillance.
相关搜索：重组人颗粒酶B，重组人GZMB，人GZMB重组蛋白，Recombinant Human Granzyme B Protein (His Tag)