产品货号：
GS4894
中文名称：
重组人UBE2I(带His标签)
英文名称：
Recombinant Human SUMO-Conjugating Enzyme UBC9, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 19.5kDa,a single non-glycosylated polypeptide chain containing 158 amino acids(a.a.)of human UBE2I/UBC9 and 8 a.a.vector sequence including 6×His tag at N-terminus.
储存条件：冷冻（-20℃）
概述
SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4.Additionally,It takes parts in catalysis the formation of poly-SUMO chains,sumoylation of FOXL2 and KAT5,and the segregation of nuclear architecture and chromosome.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人UBE2I(带His标签)，UBE2I, His-tagged, Human
储存条件：冷冻（-20℃）
概述
SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4.Additionally,It takes parts in catalysis the formation of poly-SUMO chains,sumoylation of FOXL2 and KAT5,and the segregation of nuclear architecture and chromosome.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile liquid. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Data is not available. |
| 成分 | 50mMHEPES,125 mM NaCl,1 mM DTT,10%glycerol,pH 7.6. |
| 内毒素 | Less than 1 EU/μg of rHuUBC9,His as determined by LAL method. |
| 复溶 | Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S |
相关搜索：重组人UBE2I(带His标签)，UBE2I, His-tagged, Human