产品货号：
GS4755
中文名称：
重组小鼠SHH
英文名称：
Recombinant Murine Sonic Hedgehog N-Terminus
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 19.8kDa,a single non-glycosylated polypeptide chain containing 176 amino acids.
储存条件：冷冻（-20℃）
概述
Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog,the others being desert hedgehog(DHH)and indian hedgehog(IHH).As the hh homologues,SHH has been found to have the most critical roles in development.It acts as a morphogen involved in patterning many systems,including the limb and midline structures in the brain,spinal cord,the thalamus by the zona limitans intrathalamica and the teeth.There SHH undergoes auto-processing to generate a 20 kDa(residues 24 - 197)N-terminal signaling domain(SHH-N)and a 25 kDa(residues 198 - 462)C-terminal domain with no known signaling role.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组小鼠SHH，SHH, Murine，Recombinant Murine Sonic Hedgehog N-Terminus
储存条件：冷冻（-20℃）
概述
Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog,the others being desert hedgehog(DHH)and indian hedgehog(IHH).As the hh homologues,SHH has been found to have the most critical roles in development.It acts as a morphogen involved in patterning many systems,including the limb and midline structures in the brain,spinal cord,the thalamus by the zona limitans intrathalamica and the teeth.There SHH undergoes auto-processing to generate a 20 kDa(residues 24 - 197)N-terminal signaling domain(SHH-N)and a 25 kDa(residues 198 - 462)C-terminal domain with no known signaling role.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0μg/ml. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rMuSHH as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
相关搜索：重组小鼠SHH，SHH, Murine，Recombinant Murine Sonic Hedgehog N-Terminus