产品货号：
GS4640
中文名称：
重组人SHH
英文名称：
Recombinant Human Sonic Hedgehog Protein N-Terminus
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 19.8kDa,a single non-glycosylated polypeptide chain containing 176 amino acids.
储存条件：冷冻（-20℃）
概述
Sonic Hedgehog(SHH)is one of three proteins of the Hedgehog(Hh)family,which also contains Desert Hedgehog(DHH)and Indian Hedgehog(IHH).The three members share a high degree of amino-acid sequence identity(e.g.,SHH and IHH are 93%identical).SHH is expressed in fetal intestine,liver,lung,and kidney,but not in adult tissues.The protein consists of 462 a.a.with a 23 a.a.signal peptide at N-terminus,and is further cleaved into SHH N-Terminus and C-Terminus.SHH has the most critical roles in development,acting as a morphogen involved in patterning many systems,including the limb and midline structures in the brain,spinal cord,the thalamus by the zona limitans intrathalamica and the teeth.In the absence of Sonic HedgeHog,patched receptor represses the constitutive signaling activity of smoothened.SHH-N retains all known signaling capabilities,and can be lipid-modified without receptor affinity reducing,but has more potent than the unmodified form.The rHuSHH has an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人SHH，SHH, Human
储存条件：冷冻（-20℃）
概述
Sonic Hedgehog(SHH)is one of three proteins of the Hedgehog(Hh)family,which also contains Desert Hedgehog(DHH)and Indian Hedgehog(IHH).The three members share a high degree of amino-acid sequence identity(e.g.,SHH and IHH are 93%identical).SHH is expressed in fetal intestine,liver,lung,and kidney,but not in adult tissues.The protein consists of 462 a.a.with a 23 a.a.signal peptide at N-terminus,and is further cleaved into SHH N-Terminus and C-Terminus.SHH has the most critical roles in development,acting as a morphogen involved in patterning many systems,including the limb and midline structures in the brain,spinal cord,the thalamus by the zona limitans intrathalamica and the teeth.In the absence of Sonic HedgeHog,patched receptor represses the constitutive signaling activity of smoothened.SHH-N retains all known signaling capabilities,and can be lipid-modified without receptor affinity reducing,but has more potent than the unmodified form.The rHuSHH has an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1μg/ml,corresponding to a specific activity of>1.0×103 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.4,150 mM NaCl. |
| 内毒素 | Less than 1 EU/μg of rHuSHH as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
相关搜索：重组人SHH，SHH, Human