MYLK抗体

产品货号：

Z90009

中文名称：

MYLK抗体

英文名称：

Anti-MYLK Antibody

产品规格：

50μL|100μL|150μL

发货周期：

1～3天

产品价格：

询价

基本参数
基因名：MYLK
Uniprot ID：Q15746
抗体来源：兔
克隆：多克隆
反应种属：人、大鼠、小鼠
抗体亚型：兔IgG
分子量：211KD
免疫原：E.coli-derived human MYLK recombinant protein (Position:D1441-D1709).
纯化方式：免疫原亲和纯化
抗体浓度：500μg/mL
产品形态：液体
储存液：PBS，0.02% NaN₃,1mg BSA and 50% glycerol.
保存条件：-20℃，有效期1年

产品应用

应用	稀释度*
Western blot	1:500～2000
IHC-P	1:50～400
Direct ELISA	1:100～1000

最优稀释度需要用户自己调试，此处数据仅供参考。

背景资料
Myosin light chain kinase,smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene.This gene,a muscle member of the immunoglobulin gene superfamily,encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme.This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity.This gene encodes both smooth muscle and nonmuscle isoforms.In addition,using a separate promoter in an intron in the 3' region,it encodes telokin,a small protein identical in sequence to the C-terminus of myosin light chain kinase,that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments.A pseudogene is located on the p arm of chromosome 3.Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.

应用实例

	Figure 1.Western blot analysis of MYLK using anti-MYLK antibody (Z90009).Lane 1:human SGC-7901 whole cell lysates,Lane 2:rat spleen tissue lysates,Lane 3:mouse spleen tissue lysates.anti-MYLK antigen affinity purified polyclonal antibody (Catalog # Z90009)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # ZN1926).A specific band was detected for MYLK at approximately 250KD.The expected band size for MYLK is at 211KD.

Figure 2.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of mouse lung tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.	Figure 3.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of mouse small intestine tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.

Figure 4.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of rat small intestine tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.	Figure 5.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of human placenta tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.

Figure 6.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of human rectal cancer tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.	Figure 7.IHC analysis of MYLK using anti-MYLK antibody (Z90009).MYLK was detected in paraffin-embedded section of rat lung tissue.anti-MYLK Antibody (Z90009).Biotinylated goat anti-rabbit IgG was used as secondary antibody.The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # ZN1830) with DAB as the chromogen.

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