产品货号：
Z90008
中文名称：
EPHA2抗体
英文名称：
Anti EEPHA2 Antibody
产品规格：
50μL|100μL|150μL
发货周期：
1～3天
产品价格：
询价
基本参数：
基因名：EPHA2
Uniprot ID：EPHA2(P29317)
抗体来源：兔
克隆：多克隆
抗体类型：兔IgG
反应性：人，小鼠，大鼠
分子量：125KD
免疫原：E.coli-derived human Eph receptor A2 recombinant protein (Position:M851-N970)
纯化方式：免疫亲和纯化
浓度：500μg/mL
产品形态：溶液
储存液：PBS，0.02% NaN3,1mg BSA and 50% glycerol
保存条件：-20℃可保存6个月，避免反复冻融
推荐应用：
*最优稀释度需要用户自己调试，此处数据仅供参考。
应用实例：
实例1：
Figure 1.Western blot analysis of EPHA2 using anti-EPHA2 antibody (Z90008).
Lane 1:human Hela cell lysate,
Lane 2:human U-87MG cell lysate,
Lane 3:human SHG-44 cell lysate,
Lane 4:human COLO-320 cell lysate,
Lane 5:human SK-OV-3 cell lysate,
Lane 6:human A549 cell lysate,
Lane 7:mouse HEPA1-6 cell lysate.
anti-EPHA2 antigen affinity purified polyclonal antibody (Catalog # Z90008)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # ZN1926).A specific band was detected for EPHA2 at approximately 125KD.The expected band size for EPHA2 is at 108KD.
实例2：
Figure 2.Flow cytometry analysis of A549 cell HELA(1×106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488.Unlabelled sample (Red line).
实例3：
Figure 3.ICC analysis using anti- EPHA2 antibody (Z90008).was detected in immersion fixed PC-3 cell line.Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog#ZN1991) and counterstained with DAPI (blue).
背景资料：
EPHA2(ephrin type-A receptor 2) also known as ECK,is a protein that in humans is encoded by the EPHA2 gene.This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family.Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.By somatic cell hybrid analysis and fluorescence in situ hybridization,the EPHA2 gene is mapped to chromosome 1p36.1.EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry.EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1 (CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
相关搜索：EPHA2抗体，EPHA2多抗，EPHA2兔多抗，EPHA2多克隆抗体，ECK抗体，ARCC2抗体，CTPA抗体，CTPP1抗体，CTRCT6抗体，Anti EEPHA2 Antibody
基因名：EPHA2
Uniprot ID：EPHA2(P29317)
抗体来源：兔
克隆：多克隆
抗体类型：兔IgG
反应性：人，小鼠，大鼠
分子量：125KD
免疫原：E.coli-derived human Eph receptor A2 recombinant protein (Position:M851-N970)
纯化方式：免疫亲和纯化
浓度：500μg/mL
产品形态：溶液
储存液：PBS，0.02% NaN3,1mg BSA and 50% glycerol
保存条件：-20℃可保存6个月，避免反复冻融
推荐应用：
| 应用 | 稀释度* |
| Western blot | 1:500～2000 |
| ICC/IF | 1:50～400 |
| Flow Cytometry | 1～3μg/1×106cells |
| Direct ELISA | 1:100～1000 |
*最优稀释度需要用户自己调试，此处数据仅供参考。
应用实例：
实例1：
Figure 1.Western blot analysis of EPHA2 using anti-EPHA2 antibody (Z90008).
Lane 1:human Hela cell lysate,
Lane 2:human U-87MG cell lysate,
Lane 3:human SHG-44 cell lysate,
Lane 4:human COLO-320 cell lysate,
Lane 5:human SK-OV-3 cell lysate,
Lane 6:human A549 cell lysate,
Lane 7:mouse HEPA1-6 cell lysate.
anti-EPHA2 antigen affinity purified polyclonal antibody (Catalog # Z90008)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # ZN1926).A specific band was detected for EPHA2 at approximately 125KD.The expected band size for EPHA2 is at 108KD.
实例2：
Figure 2.Flow cytometry analysis of A549 cell HELA(1×106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488.Unlabelled sample (Red line).
实例3：
Figure 3.ICC analysis using anti- EPHA2 antibody (Z90008).was detected in immersion fixed PC-3 cell line.Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog#ZN1991) and counterstained with DAPI (blue).
背景资料：
EPHA2(ephrin type-A receptor 2) also known as ECK,is a protein that in humans is encoded by the EPHA2 gene.This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family.Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.By somatic cell hybrid analysis and fluorescence in situ hybridization,the EPHA2 gene is mapped to chromosome 1p36.1.EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry.EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1 (CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
相关搜索：EPHA2抗体，EPHA2多抗，EPHA2兔多抗，EPHA2多克隆抗体，ECK抗体，ARCC2抗体，CTPA抗体，CTPP1抗体，CTRCT6抗体，Anti EEPHA2 Antibody