产品货号：
Z38459
中文名称：
HEF1/NEDD9抗体
英文名称：
Anti-HEF1/NEDD9 Antibody (Clone#2G9)
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-HEF1/NEDD9 Antibody (Clone#2G9) |
| 指标别名 | neural precursor cell expressed;developmentally down-regulated 9;CAS-L;CAS2;CASL;CASS2;HEF1;NEDD9 |
| 克隆性 | Monoclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | IF,IP,WB |
| 基因名称 | NEDD9 |
| 克隆号 | 2G9 |
| 抗体来源 | Mouse |
| 免疫原 | Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein (hHEF1,843 aa,predicted MW 92.8kDa). |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This Protein A purified antibody is directed against human HEF1 protein.The product was purified from tissue culture supernatant by chromatography.Reactivity occurs against human,mouse and rat forms of the protein.Reactivity against multiple isoforms is expected.Reactivity against homologues from other sources is not known.Specificity was determined by partial epitope mapping. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | HEF1,also known as Enhancer of filamentation 1,CRK-associated substrate-related protein,CAS-L,CasL,p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene.HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion.HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation.HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells.Integrin beta-1 stimulation leads to recruitment of various proteins including CRK,NCK and SHPTP2 to the tyrosine phosphorylated form.HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2,E12,E47 and also with p130cas.HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK),adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1.This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells.In fibroblasts,it is predominantly nuclear and in some cells is present in the Golgi apparatus.In epithelial cells,it is localized predominantly in the cell periphery with particular concentration in lamellipodia,but it is also found in the nucleus.HEF1 is widely expressed although higher levels are detected in kidney,lung,and placental tissue.HEF1 is also detected in T-cells,B-cells and diverse cell lines.HEF1 is activated upon induction of cell growth.Cell cycle-regulated processing produces four isoforms:p115,p105,p65,and p55.Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle.Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis.The p65 isoform is poorly detected.Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle. |
| Uniprot ID | Q14511 |
| 推荐稀释比 | ELISA:1:5,000 - 1:20,000 IF Microscopy:1:500 IP:1:1,000 WB:1:5,000 |
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