产品货号：
Z31904
中文名称：
CD3E (activation epitope) Purified抗体
英文名称：
Anti-CD3E (activation epitope) Purified Antibody (Clone#APA1/1)
产品规格：
0.1mg(C100)
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-CD3E (activation epitope) Purified Antibody (Clone#APA1/1) |
| 指标别名 | CD3;CD3 epsilon;CD3E;CD3-epsilon;T3E;TCRE |
| 克隆性 | Monoclonal |
| 检验物种 | mouse,human |
| 应用范围 | FCM,IP,WB,IHC-F,ICC |
| 基因名称 | CD3E |
| 克隆号 | APA1/1 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG1 |
| 免疫原 | Purified human CD3 proteins isolated from thymus.The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon.Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70,which initiates the signaling cascade produced by T-cell activation.APA1/1 provides the earliest known marker for TCR-mediated T cell activation. |
| 计算分子量 | 23kDa |
| 成分 | Phosphate buffered saline (PBS),pH7.4,15mM sodium azide |
| 纯化方式 | Purified by protein-A affinity chromatography. |
| 浓度 | 1mg/mL |
| 产品形态 | 溶液 |
| 保存条件 | Store at 2～8℃.Do not freeze. |
| 背景资料 | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma,CD3 delta,CD3 epsilon and CD3 zeta.These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11.The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules.This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits.CD3 may play a role in TCR-induced growth arrest,cell survival and proliferation. |
| Uniprot ID | P07766 |
| 基因名全称 | CD3 antigen,epsilon polypeptide |
| 蛋白名全称 | T-cell surface glycoprotein CD3 epsilon chain |
| 推荐稀释比 | Flow cytometry:Intracellular staining;recommended dilution:1～4μg/mL;positive control:human T cells stimulated with anti-CD3 (MEM-57) antibody (1μg/mL).Sample preparation:At the end of stimulation of T cells,perform staining of surface markers (if required) in PBS + 0.1% BSA for 20min.on ice.Wash with PBS and fix with 2% formaldehyde,30min on ice.Wash with PBS and incubate in PBS + 0,1% saponine,5min.RT.Incubate the cells in PBS + 1% BSA + 0.03% saponine,15min.on ice.Incubate with fluorescence-labeled APA1/1 antibody (1～2μg/mL) in PBS + 1% BSA + 0.03% saponine in dark,20min.RT.Wash with PBS + 1% BSA + 0.03% saponine,resuspend in PBS. Immunocytochemistry:Fixed and permeabilised cells.The antibody can distinguish TCR-stimulated from non-stimulated cells. |
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