产品货号:
Z31800
中文名称:
Cytokeratin 5/KRT5抗体
英文名称:
Anti-Cytokeratin 5/KRT5 Antibody (Clone#9H2F8)
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-Cytokeratin 5/KRT5 Antibody (Clone#9H2F8) |
指标别名 | 58kDa cytokeratin;Cell and organelle markers;CK 5;CK5;Cytokeratin 5;Cytoskeleton Marker;K5;keratin 5;KRT5;KRT5A;Type II keratin Kb5 |
克隆性 | Monoclonal |
检验物种 | human |
应用范围 | WB,IHC,ICC/IF,FCM |
基因名称 | KRT5 |
克隆号 | 9H2F8 |
抗体来源 | Mouse |
抗体类型 | IgG2a |
免疫原 | A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5 (286-317aa KVELEAKVDALMDEINFMKMFFDAELSQMQTH),different from the related mouse sequence by one amino acid,and identical to the related rat sequence. |
实际分子量 | 62KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | protein G purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | Cytokeratin 5,also known as KRT5,K5,or CK5,is a protein that is encoded in humans by the KRT5 gene.The protein encoded by this gene is a member of the keratin gene family.The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14.Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex.The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
Uniprot ID | P13647 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
基因名全称 | keratin 5 |
蛋白名全称 | Keratin,type II cytoskeletal 5 |
推荐稀释比 | Western blot(WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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