产品货号：
Z31460
中文名称：
EIF6抗体
英文名称：
Anti-EIF6 Antibody (Clone#2I11)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-EIF6 Antibody (Clone#2I11) |
| 指标别名 | b(2)gcn;B(2)GCN homolog;CAB;eIF 6;EIF3A;EIF6;gcn;integrin beta 4 binding protein;ITGB4BP;p27(BBP);p27BBP |
| 克隆性 | Monoclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,FCM |
| 基因名称 | EIF6 |
| 克隆号 | 2I11 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2a |
| 免疫原 | E.coli-derived human EIF6 recombinant protein (Position:N66-T210).Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6. |
| 实际分子量 | 26KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | EIF6 (Eukaryotic Translation Initiation Factor 6),also called EIF3A or ITGB4BP,is a human gene.By fluorescence in situ hybridization,Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2.Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6.In the cytoplasm,EIF6 is bound to free 60S but not to 80S subunits.Furthermore,EIF6 interacts in the cytoplasm with RACK1,a receptor for activated protein kinase C.Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo.Eif6-null mouse embryos were lethal at preimplantation.Heterozygous mice had 50% reduction of eIF6 levels in all tissues,and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression. |
| Uniprot ID | P56537 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
相关搜索：EIF6抗体，EIF6单抗，Anti-EIF6 Antibody (Clone#2I11)