产品货号：
Z30198
中文名称：
CDC25B抗体
英文名称：
Anti-CDC25B Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-CDC25B Antibody |
| 指标别名 | CDC25B;CDC25HU2;M phase inducer phosphatase 2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,rat |
| 应用范围 | WB,IHC |
| 基因名称 | CDC25B |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25B(541-559aa DYRPMNHEAFKDELKTFRL),different from the related mouse sequence by three amino acids. |
| 实际分子量 | 65KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase,the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation.The latter function is carried out by a specific phosphatase,CDC25.At least 3 human CDC25 genes code for the A,B,and C forms of CDC25.CDC25B is mapped to 20p13.P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation.Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation.In vitro,p38 binds and phosphorylates CDC25B at serines 309 and 361,and CDC25C at serine-216;phosphorylation of these residues is required for binding to 14-3-3 proteins.In vivo,inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation,and mutation of this site is sufficient to inhibit the checkpoint initiation.Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation. |
| Uniprot ID | P30305 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | cell division cycle 25 homolog B(S.pombe) |
| 蛋白名全称 | M-phase inducer phosphatase 2 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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