产品货号：
Z28941
中文名称：
UBE2Q2抗体
英文名称：
Anti-UBE2Q2 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-UBE2Q2 Antibody |
| 指标别名 | UBCi;UBE2Q2;Ubiquitin carrier protein Q2;Ubiquitin protein ligase Q2 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | UBE2Q2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2 (83-123aa LERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVEMLDQ),different from the related mouse sequence by four amino acids. |
| 实际分子量 | 50KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | UBE2Q2 is identified as a putative ubiquitin-conjugating enzyme (E2) in a microarray screen for mitotic regulatory proteins.Its gene is mapped to 15q24.2.UBE2Q2 can covalently bind ubiquitin on the active site cysteine within the UBC domain.Inhibition of UBE2Q2 in HeLa cells causes an early mitotic arrest and increases cytotoxicity when the cells are treated with microtubule-inhibiting agents (MIAs).Changes in cell cycle progression and viability are not observed in the absence of MIA treatment,indicating that UBE2Q2 is involved in the response to MIAs rather than performing a more general function in mitosis.Moreover,inhibition of the UBE2Q2 protein causes cells to undergo a prolonged prophase arrest,suggesting that UBE2Q2 normally functions to antagonize an early mitotic checkpoint.Finally,inhibition of UBE2Q2 also sensitizes cells to the cytotoxic effects of MIAs through caspase-mediated apoptosis that is correlated with PARP1 cleavage. |
| Uniprot ID | Q8WVN8 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | ubiquitin conjugating enzyme E2Q family member 2 |
| 蛋白名全称 | Ubiquitin-conjugating enzyme E2 Q2 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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