产品货号：
Z27139
中文名称：
SPARC抗体
英文名称：
Anti-SPARC Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-SPARC Antibody |
| 指标别名 | Basement membrane protein 40;BM 40;ON;Osteonectin;SPARC |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC |
| 基因名称 | SPARC |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human SPARC(284-303aa DEWAGCFGIKQKDIDKDLVI). |
| 实际分子量 | 35KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | SPARC,secreted protein acidic and rich in cysteine,also known as Osteonectin is a protein that in humans is encoded by the SPARC gene.The human SPARC gene is 26.5kb long,and contains 10 exons and 9 introns and is located on chromosome 5q31-q33.SPARC is a glycoprotein of 40kD.SPARC is an acidic,cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains:1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I),2) a cysteine- rich(domain II),3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV).Osteonectin is a glycoprotein in the bone that binds sodium.It is secreted by osteoblasts during bone formation,initiating mineralization and promoting mineral crystal formation.Osteonectin also shows affinity for collagen in addition to bone mineral calcium.A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found. |
| Uniprot ID | P09486 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | secreted protein,acidic,cysteine-rich(osteonectin) |
| 蛋白名全称 | SPARC |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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