产品货号：
Z27049
中文名称：
SMAD1抗体
英文名称：
Anti-SMAD1 Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-SMAD1 Antibody |
| 指标别名 | BSP 1;BSP1;hSMAD1;JV4 1;JV41;MAD homolog 1;Mad related protein 1;MADH1;MADR1;Mothers against DPP homolog 1;SMAD 1;SMAD family member 1;SMAD1 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,WB |
| 基因名称 | SMAD1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein. |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue.The product was affinity purified from monospecific antiserum by immunoaffinity purification.Antiserum was first purified against the phosphorylated form of the immunizing peptide.The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.Reactivity occurs against human SMAD1 pS206 protein and the antibody is specific for the phosphorylated form of the protein.Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot.A BLAST analysis was used to suggest 100% cross-reactivity with SMAD1 from human,Duckbill platypus,Drosophila,mouse,opossum,chimpanzee,bonobo,orangutan,gorilla,macaque,cattle,sheep,pig,rat,horse,salmon,chicken,and dog based on the sequence homology with the immunogen.Reactivity against homologues from other sources is not known. |
| 浓度 | 1.31mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | SMAD1 is also known Mothers Against Decapentaplegic homolog 1,Mothers against DPP homolog 1,hSMAD-3,JV4-1,Transforming growth factor-Beta-Signaling Protein 1 or BSP1.SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.SMAD1,as a transcriptional modulator,is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD).BMPs are involved in a range of biological activities including cell growth,apoptosis,morphogenesis,development and immune responses.SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling.In response to BMP ligands,SMAD1 can be phosphorylated (other sites besides the most prominent of S206,are S187,S195,and S214).S-206 is phosphorylated by ERK in response to mitogenic growth factors,or by recombinant ERK in vitro;this can be tested by treating cells with EGF or in cancer cells where Ras is activated.The phosphorylated form of this protein forms a complex with SMAD4,which is important for its function in the transcription regulation.This protein is also a target for SMAD-specific E3 ubiquitin ligases,such as SMURF1 and SMURF2,and undergoes ubiquitination and proteasome-mediated degradation. |
| Uniprot ID | Q15797 |
| 推荐稀释比 | ELISA:1:25,000 - 1:30,000 IP:2μg WB:1:500 |
相关搜索：SMAD1抗体，SMAD1多抗，Anti-SMAD1 Antibody