产品货号：
Z27045
中文名称：
SMAD2抗体
英文名称：
Anti-SMAD2 Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-SMAD2 Antibody |
| 指标别名 | hMAD 2;hSMAD2;JV18;JV18 1;MAD homolog 2;Mad related protein 2;MADH2;MADR2;Mothers against DPP homolog 2;SMAD 2;SMAD family member 2;SMAD2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,monkey |
| 应用范围 | ELISA,WB |
| 基因名称 | SMAD2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein. |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This affinity purified antibody is directed against human Smad2 protein.The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human,mouse and rat based on 100% homology with the immunizing sequence.Reactivity against homologues from other sources is not known.?Also,the antibody is Smad2 specific,and reactivity to other Smad proteins (specifically Smad1,Smad3,Smad4,and Smad7) is not detected in over-expressed cell lysates (Personal Communication,Kathleen Flanders,CCR-NCI,Bethesda,MD). |
| 浓度 | 0.55mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Smad2 (also known as Mothers against decapentaplegic homolog 2,Mothers against DPP homolog 2,Mad2,hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.elegans gene Sma.SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.This protein mediates the signal of the transforming growth factor (TGF)-beta,and thus regulates multiple cellular processes,such as cell proliferation,apoptosis,and differentiation.This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.In response to TGF-beta signal,this protein is phosphorylated by the TGF-beta receptors.The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus,where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase,and mediates the signal from the activin.Alternatively,spliced transcript variants have been observed for this gene.SMAD2 may act as a tumor suppressor in colorectal carcinoma.It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.SMAD2 may be associated with diseases such as Keloids and Ureteral Disease.Anti-SMAD2 Antibody is useful for researchers interested in SMAD pathways,transcription factor activity,sequence-specific DNA binding,and cancer research. |
| Uniprot ID | Q15796 |
| 推荐稀释比 | ELISA:1:100,000 IP:User Optimized WB:1:1,000 - 1:3,000 |
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