产品货号：
Z24368
中文名称：
Prohibitin 2/PHB2抗体
英文名称：
Anti-Prohibitin 2/PHB2 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Prohibitin 2/PHB2 Antibody |
| 指标别名 | BAP;Bap37;D prohibitin;p22;PHB2;PNAS 141;prohibitin 2;REA |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | PHB2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human REA/PHB2 recombinant protein (Position:M1-K299). |
| 实际分子量 | 33KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | PHB2(Prohibitin 2),also called Repressor of Estrogen Receptor Activity(REA),is a protein that in humans is encoded by the PHB2 gene.The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12.Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells.When coexpressed with wildtype ER-alpha or ER-beta(ESR2),REA suppressed activation of a reporter gene in a dose-dependent manner.REA had no effect on reporter activity in the absence of liganded ER,and it had no effect on the transcriptional activities of other hormone receptors.Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity. |
| Uniprot ID | Q99623 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | prohibitin 2 |
| 蛋白名全称 | Prohibitin-2 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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