产品货号：
Z24005
中文名称：
POLR2A抗体
英文名称：
Anti-POLR2A Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-POLR2A Antibody |
| 指标别名 | hRPB220;hsRPB1;Pol II;POLR2;POLR2A;POLRA;RNA polymerase II subunit B1;RPB1;RPBh1;RpIILS;RPO2;RPOL2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,monkey,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | POLR2A |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human RNA polymerase II/POLR2A recombinant protein (Position:D10-E321). |
| 实际分子量 | 225KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | DNA-directed RNA polymerase II subunit RPB1,also known as RPB1,is an enzyme that in humans is encoded by the POLR2A gene.It is mapped to 17p13.1.This gene encodes the largest subunit of RNA polymerase II,the polymerase responsible for synthesizing messenger RNA in eukaryotes.The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity.These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.In addition,this subunit,in combination with several other polymerase subunits,forms the DNA binding domain of the polymerase,a groove in which the DNA template is transcribed into RNA. |
| Uniprot ID | P24928 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
| 基因名全称 | RNA polymerase II subunit A |
| 蛋白名全称 | DNA-directed RNA polymerase II subunit RPB1 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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