产品货号：
Z23918
中文名称：
Paxillin/PXN抗体
英文名称：
Anti-Paxillin/PXN Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Paxillin/PXN Antibody |
| 指标别名 | paxillin;PXN |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IF,IHC-F,ICC/IF,FCM |
| 基因名称 | PXN |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin(456-472aa HEKDGKAYCRKDYFDMF),identical to the related mouse and rat sequences. |
| 实际分子量 | 65KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge.Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions,it is presumed through a direct association with the cytoplasmic tail of beta-integrin.The N-terminal region of paxillin is rich in protein–protein interaction sites.The proteins that bind to paxillin are diverse and include protein tyrosine kinases,such as Src and FAK,structural proteins,such as vinculin and actopaxin,and regulators of actin organization,such as COOL/PIX and PKL/GIT.Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation,creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains,a proline-rich domain containing a consensus SH3-binding site,and 3 potential SH2-binding sites.On Northern blots,paxillin was expressed as a 3.7-kb mRNA in all tissues tested. |
| Uniprot ID | P49023 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section (IHC-F) 1:50～400 Immunofluorescence (IF) 1:50～400 Immunocytochemistry/Immunofluorescence(ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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