产品货号：
Z23861
中文名称：
PKC Eta/PRKCH抗体
英文名称：
Anti-PKC Eta/PRKCH Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PKC Eta/PRKCH Antibody |
| 指标别名 | protein kinase C eta;PKC-L;PKCL;PRKCL;nPKC-eta;PRKCH |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC,FCM |
| 基因名称 | PRKCH |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human PKC eta recombinant protein (Position:R30-D389).Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta,respectively. |
| 实际分子量 | 78KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | PRKCH is also known as PKC eta.Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol.PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.Also,PKC family members serve as major receptors for phorbol esters,a class of tumor promoters.Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.The protein encoded by this gene is one of the PKC family members.It is a calcium-independent and phospholipids-dependent protein kinase.And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus.This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA).It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. |
| Uniprot ID | P24723 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | protein kinase C,eta |
| 蛋白名全称 | Protein kinase C eta type |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry in fixed cells(ICC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 23 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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