产品货号：
Z23542
中文名称：
Osteopontin/SPP1抗体
英文名称：
Anti-Osteopontin/SPP1 Antibody
产品规格：
200μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Osteopontin/SPP1 Antibody |
| 指标别名 | BNSP;Bone sialoprotein 1;BSPI;ETA 1;Nephropontin;OPN;Osteopontin;secreted phosphoprotein 1;SPP 1;SPP1;Urinary stone protein;Uropontin |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,IHC,WB |
| 基因名称 | SPP1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide,from the human osteopontin protein,conjugated to KLH using maleimide. |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | Osteopontin is directed against human osteopontin.The antibody recognizes the full-length osteopontin protein (which runs at 66kDa on westerns),as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN.The 32kDa MMP-cleaved C-fragment is recognized,but not the 40kDa N-terminal fragment.Reactivity is reported to occur with osteopontin from swine,dog,mouse and rat. |
| 浓度 | 95mg/mL by Refractometry |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Anti-Osteopontin Antibody recognizes Osteopontin (OPN) which is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors.OPN is multifunctional,with activities in cell migration,cell survival,inhibition of calcification,regulation of immune cell function,and control of tumor cell phenotype.The gene encoding OPN is called spp1.Targeting this gene has revealed that while OPN is not necessary for normal embryonic development,fertility,and health under pathogen-free conditions,loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury,viral,and bacterial infection,bone remodeling,and tumor growth.The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress.Interestingly,several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN.OPN has recently been shown to be a novel substrate for two MMPs,MMP-3 (stromelysin-1) and MMP-7 (matrilysin).There are three cleavage sites for MMP-3 in human OPN,two of which are also cleaved by MMP-7 (see cleavage diagram).Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN.In addition,inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells.It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.Osteopontin can be responsible for diseases such as lung and prostate cancers,nephrolithiasis,hepatocellular carcinoma,osteoporosis and arteriosclerosis.Anti-Osteopontin is useful for researchers interested in Stem Cell and Extracellular Matrix Antibodies. |
| Uniprot ID | P10451 |
| 推荐稀释比 | ELISA:1:5,000 - 1:20,000 IHC:1:100 - 1:300 IP:1:100 WB:1:500 - 1:2,000 |
相关搜索：Osteopontin/SPP1抗体，Osteopontin抗体，SPP1多抗，Anti-Osteopontin/SPP1 Antibody