产品货号：
Z23304
中文名称：
PCNA抗体
英文名称：
Anti-PCNA Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PCNA Antibody |
| 指标别名 | PCNA |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,FCM,ELISA |
| 基因名称 | PCNA |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human PCNA recombinant protein (Position:M1-S261). |
| 实际分子量 | 36KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication.It is mapped to 20p12.3.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Uniprot ID | P12004 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (ELISA)： 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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