产品货号：
Z21474
中文名称：
NDUFB10抗体
英文名称：
Anti-NDUFB10 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NDUFB10 Antibody |
| 指标别名 | CI PDSW;Complex I PDSW;NDUFB10;PDSW |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | NDUFB10 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human NDUFB10 recombinant protein (Position:D36-S172). |
| 实际分子量 | 22KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 is an enzyme that in humans is encoded by the NDUFB10 gene.The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is not directly involved in catalysis.Mammalian complex I is composed of 45 different subunits.It locates at the mitochondrial inner membrane.This protein complex has NADH dehydrogenase activity and oxidoreductase activity.It transfers electrons from NADH to the respiratory chain.The immediate electron acceptor for the enzyme is believed to be ubiquinone.Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.[7] Initially,NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2.The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ),which is reduced to ubiquinol (CoQH2).The flow of electrons changes the redox state of the protein,resulting in a conformational change and pK shift of the ionizable side chain,which pumps four hydrogen ions out of the mitochondrial matrix. |
| Uniprot ID | O96000 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | NADH:ubiquinone oxidoreductase subunit B10 |
| 蛋白名全称 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 |
| 推荐稀释比 | Western blot (WB) 1:1000～5000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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