产品货号:
Z21387
中文名称:
NEDD4抗体
英文名称:
Anti-NEDD4 Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-NEDD4 Antibody |
指标别名 | KIAA0093;NEDD 4;NEDD4;NEDD4 1;RPF1 |
克隆性 | Polyclonal |
检验物种 | human,rat |
应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
基因名称 | NEDD4 |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | E.coli-derived human NEDD4 recombinant protein (Position:M1-A736). |
实际分子量 | 105~150KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | E3 ubiquitin-protein ligase NEDD4,also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4),is an enzyme that in humans is encoded by the NEDD4 gene.This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation.The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and,a C-terminal HECT ubiquitin ligase catalytic domain.It plays critical role in the regulation of a number of membrane receptors,endocytic machinery components and the tumor suppressor PTEN. |
Uniprot ID | P46934 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
基因名全称 | NEDD4 E3 ubiquitin protein ligase |
蛋白名全称 | E3 ubiquitin-protein ligase NEDD4 |
推荐稀释比 | Western blot (WB) 1:500~2000 Immunohistochemistry (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100~1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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