产品货号：
Z21382
中文名称：
NEDD8抗体
英文名称：
Anti-NEDD8 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NEDD8 Antibody |
| 指标别名 | cul nedd8;Nedd 8;NEDD8;Neddylin;Ubiquitin like protein Nedd8 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IHC-F,ICC/IF,FCM |
| 基因名称 | NEDD8 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence in the middle region of human NEDD8 (20-60aa TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK),identical to the related mouse and rat sequences. |
| 实际分子量 | 10KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | NEDD8 is a protein that in humans is encoded by the NEDD8 gene.Human NEDD8 shares 60% amino acid sequence identity to ubiquitin.The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases,which are active only when neddylated.Their NEDDylation is critical for the recruitment of E2 to the ligase complex,thus facilitating ubiquitin conjugation.NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation. |
| Uniprot ID | Q15843 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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