产品货号:
Z21359
中文名称:
NDUFA1抗体
英文名称:
Anti-NDUFA1 Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
| 抗体名称 | Anti-NDUFA1 Antibody |
| 指标别名 | NADH:ubiquinone oxidoreductase subunit A1;CI-MWFE;MC1DN12;MWFE;ZNF183;NDUFA1 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC,IHC-F |
| 基因名称 | NDUFA1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID),different from the related rat and mouse sequences by one amino acid. |
| 计算分子量 | 8KD |
| 实际分子量 | 8KD |
| 成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX,1),also called MWFE,B.TAURUS,HOMOLOG OF,encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase,also known as mitochondrial complex I.The NDUFA1 gene is mapped to chromosome Xq24.The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain,likely to be a transmembrane domain,and a C-terminal hydrophilic domain.And the NDUFA1 gene contains 3 exons and spans about 5.0kb of genomic DNA.Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity.The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals.The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation.Thus the NDUFA1 peptide may also participate in that function. |
| Uniprot ID | O15239 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | NADH dehydrogenase(ubiquinone) 1 alpha subcomplex,1,7.5kDa |
| 蛋白名全称 | NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 |
| 推荐稀释比 | Western blot (WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunohistochemistry in frozen section (IHC-F) 1:50~400 Immunocytochemistry 1:50~400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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