产品货号：
Z21082
中文名称：
Mouse IL18抗体
英文名称：
Anti-Mouse IL18 Antibody
产品规格：
500μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Mouse IL18 Antibody |
| 指标别名 | Iboctadekin;IFN gamma inducing factor;IGIF;IL 1 gamma;IL 18;IL 1g;IL18;IL-18;IL1F4;Interleukin 1 gamma;Interleukin 18;Mature IL18;pre IL18;Pre-IL18 |
| 克隆性 | Polyclonal |
| 检验物种 | mouse |
| 应用范围 | IF,IHC |
| 基因名称 | IL18 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli. |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2 |
| 纯化方式 | This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology.This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays.This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18.In general,this antibody also detects rat IL-18 in the same formats using similar dilutions.?A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF).The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/macrophages,Kupffer cells,keratinocytes,glucocorticoid-secreting adrenal cortex cells,osteoblasts and dendritic cells.IL-18 is a 24kDa,non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1.IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature,bioactive,18kDa molecule.In both the mature and propeptide forms,IL-18 shows 64% aa sequence identity from mouse to human.IL-18 does not appear to show any primary sequence similarity to any other known cytokines.Rat IL-18 has also been isolated,and found to be 194 aa in length with a 91% aa sequence identity to mouse IL-18.Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC.With respect to human T cells,IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway.Human IL-18 can also inhibit the synthesis of IgE by B cells.Thus,IL-18 plays an important role in immunological and inflammatory reactions.Currently,the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants. |
| Uniprot ID | P70380 |
| 推荐稀释比 | ELISA:1:1,000 - 1:5,000 IHC:User Optimized IF Microscopy:1:50～1:200 WB:1:500 - 1:2,000 |
相关搜索：Mouse IL18抗体，Mouse IL18多抗，Anti-Mouse IL18 Antibody