产品货号:
Z20420
中文名称:
MGMT抗体
英文名称:
Anti-MGMT Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-MGMT Antibody |
指标别名 | MGMT |
克隆性 | Polyclonal |
检验物种 | human |
应用范围 | WB,IHC,ICC/IF,FCM |
基因名称 | MGMT |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | E.coli-derived human MGMT recombinant protein (Position:D2-N207).Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT,respectively. |
实际分子量 | 21KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | O6-alkylguanine DNA alkyltransferase (also known as AGT,MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene.MGMT is mapped to 10q26.3.This gene is crucial for genome stability.It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription.It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival. |
Uniprot ID | P16455 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
基因名全称 | O-6-methylguanine-DNA methyltransferase |
蛋白名全称 | Methylated-DNA--protein-cysteine methyltransferase |
推荐稀释比 | Western blot(WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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