产品货号：
Z19901
中文名称：
LC3B/MAP1LC3B抗体
英文名称：
Anti-LC3B/MAP1LC3B antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-LC3B/MAP1LC3B antibody |
| 指标别名 | LC3;LC3B;MAP1A/1BLC3;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;MAP1ALC3;MAP1LC3B |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,IP,FCM(Intracellular),Direct ELISA |
| 基因名称 | MAP1LC3B |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human LC3B/MAP1LC3B recombinant protein (Position:M1-G120). |
| 实际分子量 | 15～18KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | LC3B,also named as MAP1LC3B,MAP1A/1BLC3,belongs to the MAP1 LC3 family.It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins,which are involved in microtubule assembly and important for neurogenesis.In cell biology,autophagy,or autophagocytosis,is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery.It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes.Two forms of LC3,called LC3-I (17～19kd) and -II(14～16kd),were produced post-translationally in various cells.LC3-I is cytosolic,whereas LC3-II is membrane bound.The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form,LC3-I.This is activated by APG7L/ATG7,transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form,LC3-II.The amount of LC3-II is correlated with the extent of autophagosome formation.LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.MAP1LC3 has 3 isoforms MAP1LC3A,MAP1LC3B and MAP1LC3C.MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue,like their rat counterpart,MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.This antibody is specific to LC3B. |
| Uniprot ID | Q9GZQ8 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 ImmunoPrecipitation (IP) 1:50 Flow Cytometry(Intracellular) 1～3μg/1×106 cells Direct ELISA 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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