产品货号：
Z18826
中文名称：
IL-1 alpha/IL1A抗体
英文名称：
Anti-IL-1 alpha/IL1A Antibody
产品规格：
1mg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-IL-1 alpha/IL1A Antibody |
| 指标别名 | Hematopoietin 1;IL 1 alpha;IL 1A;IL1;IL-1;IL1 ALPHA;IL-1 alpha;IL1A;IL1F1;IL-1α;Interleukin 1 alpha;interleukin 1,alpha;IL-1A |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB |
| 基因名称 | IL1A |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.?The MW of the recombinant IL-1a was 17,000.?This is the cleavage site generated by the IL-1b converting enzyme (ICE,capase-1). |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays.In general,this antibody also detects primate IL-1a in the same formats using similar dilutions.The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a.In ELISA formats and other immunoreactive assays,this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples.Unlike the IL-1b precursor,the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form.?The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly.The precursor of IL-1a,unlike that of IL-1b,is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell.This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays.It does not neutralize the biological activity IL-1b.It does not neutralize the biological activity of mouse,rat or rabbit IL-1a.It will neutralize primate IL-a.For neutralization,it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4hours before being tested.A control of similarly diluted normal rabbit IgG is recommended.This antibody can be used for FACS analysis.Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Interleukin 1 alpha is produced by activated macrophages.Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research. |
| Uniprot ID | P01583 |
| 推荐稀释比 | ELISA:1:1,000 - 1:5,000 Flow Cytometry:User Optimized IHC:1:100 to 1:200 WB:1:500 - 1:2,000 |
相关搜索：IL-1 alpha/IL1A抗体，IL-1 alpha抗体，IL1A多抗，Anti-IL-1 alpha/IL1A Antibody