产品货号:
Z18725
中文名称:
Histone H2A.X抗体
英文名称:
Anti-Histone H2A.X Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-Histone H2A.X Antibody |
指标别名 | H2A histone family;member X;H2A.X;H2A/X;H2AFX;H2AX;Histone H2A.x |
克隆性 | Polyclonal |
检验物种 | human,mouse,rat |
应用范围 | WB,IHC,ICC/IF,FCM(Intracellular),Direct ELISA |
基因名称 | H2AX |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | E.coli-derived human Histone H2A.X/H2AFX recombinant protein (Position:S2-S140). |
实际分子量 | 15KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.Two molecules of each of the four core histones (H2A,H2B,H3,and H4) form an octamer,around which approximately 146bp of DNA is wrapped in repeating units,called nucleosomes.The linker histone,H1,interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.This gene encodes a replication-independent histone that is a member of the histone H2A family,and generates two transcripts through the use of the conserved stem-loop termination motif,and the polyA addition motif. |
Uniprot ID | P16104 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
基因名全称 | H2A histone family member X |
蛋白名全称 | Histone H2AX |
推荐稀释比 | Western blot (WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow Cytometry(Intracellular) 1~3μg/1×106 cells Direct ELISA 1:100~1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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