产品货号：
Z18284
中文名称：
IL-1 beta/IL1B抗体
英文名称：
Anti-IL-1 beta/IL1B Antibody
产品规格：
1mg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-IL-1 beta/IL1B Antibody |
| 指标别名 | IL1 beta;Catabolin;IL 1;IL 1 beta;IL1 BETA;IL-1 beta;IL1- beta;IL1B;IL1beta;IL1F2;IL-1β;Interleukin 1 beta;interleukin 1;beta;Pro IL-1β;IL 1B;IL-1B |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,IHC,WB |
| 基因名称 | IL1B |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This antibody was prepared by repeated immunizations with recombinant human IL-1?produced in E.coli.?The MW of the recombinant 153 aa IL-1?was 17kDa with the N-terminal amino acid at position alanine 117.?This cleavage site is generated by the IL-1?converting enzyme (ICE,capase-1). |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2 |
| 纯化方式 | This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.This antibody is primarily directed against mature,17,000 MW human IL-1?and is useful in determining its presence in various assays.In general,this antibody also detects primate IL-1?in the same formats using similar dilutions.The antiserum does not recognize human IL-1汐.In ELISA formats and other immunoreactive assays,this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1?containing samples but will primarily detect all of the 17,000 MW mature molecule.However,in immunoblot analysis of natural cell products or human body fluids,the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1?precursor molecule.Denatured 31,000 precursor IL-1?will be recognized by this antibody but often migrates as a 35,000 MW band.This is due to the unfolding of the denatured precursor IL-1?exposing epitopes not exposed in the natural state.In immunoblots,depending on the number of cells,the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1?precursor in lysates. |
| 浓度 | 2.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | IL-1 beta (also known as Interleukin-1 beta,IL-1?and catabolin) is produced by activated macrophages.IL-1 stimulates thymocyte proliferation by inducing IL-2 release,B-cell maturation and proliferation,and fibroblast growth factor activity.IL-1 proteins are involved in the inflammatory response,being identified as endogenous pyrogens,and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.IL-1?is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
| Uniprot ID | P01584 |
| 推荐稀释比 | ELISA:1:500 - 1:2,000 Flow Cytometry:User Optimized IHC:1:100 - 1:200 IP:1:400 - 1:800 WB:1:1,000 |
相关搜索：IL-1 beta/IL1B抗体，IL-1 beta抗体，IL1B多抗，Anti-IL-1 beta/IL1B Antibody