产品货号：
Z17442
中文名称：
H2AX (Phospho-S139)抗体
英文名称：
Anti-H2AX (Phospho-S139) Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-H2AX (Phospho-S139) Antibody |
| 指标别名 | H2A histone family;member X;H2A.X;H2A/X;H2AFX;H2AX;Histone H2A.x |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,WB |
| 基因名称 | H2AX |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein. |
| 计算分子量 | 15kDa |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | H2AX pS139 is directed against the phosphorylated form of human H2AX protein at the pS139 residue.The product was affinity purified from monospecific antiserum by immunoaffinity purification.Antiserum was first purified against the phosphorylated form of the immunizing peptide.The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.Reactivity with non-phosphorylated human H2AX is minimal by ELISA and western blot.A BLAST analysis was used to suggest 100% cross-reactivity with H2AX from human based on the sequence homology with the immunogen.Reactivity against homologues from other sources is not known. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store Histone H2A.X (phospho S139) antibody at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA.Histones play a central role in transcription regulation,DNA repair,DNA replication and chromosomal stability.DNA is wrapped around histone-groups,consisting of the core histones H2A,H2B,H3 and H4.As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139,called gamma-H2AX.ATM,ATR and PRKDCs,kinases of the PI3-family,are responsible for this phosphorylation.The modification can happen accidentally during replication fork collapse,exogenous genotoxic agents,may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes,in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination.Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks,but is not required for the recruitment of proteins to the site of DSBs.Gamma-H2AX is a sensitive target for looking at DSBs in cells.Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair.The role of the phosphorylated form of the histone in DNA repair is under.Anti-H2AX pS139 is ideal for researched interested in Histones,DNA Damage and Repair,and Epigenetics. |
| Uniprot ID | P16104 |
| 基因名全称 | Histone H2AX |
| 推荐稀释比 | ELISA:1:5,000 WB:1:1,000 |
相关搜索：H2AX (Phospho-S139)抗体，H2AX (Phospho-S139)多抗，Anti-H2AX (Phospho-S139) Antibody