产品货号：
Z17204
中文名称：
GLI2抗体
英文名称：
Anti-GLI2 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-GLI2 Antibody |
| 指标别名 | GLI family zinc finger 2;CJS;HPE9;PHS2;THP1;THP2;GLI2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ELISA |
| 基因名称 | GLI2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human GLI2 recombinant protein (Position:K721-D1457). |
| 实际分子量 | 210KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | GLI2 (Gli-Kruppel Family Member 2),also called ONCOGENE GLI2,is a protein that in humans is encoded by the GLI2 gene.Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links,which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr).The Drosophila gene Kr is a member of the gap class of segmentation genes;thoracic and anterior abdominal segments fail to form in Kr mutant embryos.By fluorescence in situ hybridization,Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14.Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo:overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas,which in humans are associated with constitutive hedgehog signaling. |
| Uniprot ID | P10070 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (ELISA)： 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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