产品货号：
Z15740
中文名称：
Cyclin B1/CCNB1抗体
英文名称：
Anti-Cyclin B1/CCNB1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Cyclin B1/CCNB1 Antibody |
| 指标别名 | G2/mitotic specific cyclin B1 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC-F,ICC/IF,FCM |
| 基因名称 | CCNB1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human Cyclin B1 recombinant protein (Position:M1-V433).Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1,respectively. |
| 实际分子量 | 55KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | CCNB also known as Cyclin B1,is a protein that in humans is encoded by the CCNB1 gene,and it is mapped to 5q13.2.The protein encoded by this gene is a regulatory protein involved in mitosis.The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF).Two alternative transcripts have been found,a constitutively expressed transcript and a cell cycle-regulated transcript,that is expressed predominantly during G2/M phase.The different transcripts result from the use of alternate transcription initiation sites.CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis.Its activation is well-regulated,and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated,it is not deactivated. |
| Uniprot ID | P14635 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | cyclin B1 |
| 蛋白名全称 | G2/mitotic-specific cyclin-B1 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in frozen section (IHC-F) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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