产品货号：
Z15682
中文名称：
Collagen Type II/COL2A1抗体
英文名称：
Anti-Collagen Type II/COL2A1 Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Collagen Type II/COL2A1 Antibody |
| 指标别名 | Alpha 1 type II collagen;ANFH;AOM;COL11A3;COL2A1;Col2α1;Collagen alpha 1(II) chain;Collagen Type II;collagen;type II;alpha 1;SEDC |
| 克隆性 | Polyclonal |
| 检验物种 | bovine,human,mouse,sheep,rat |
| 应用范围 | Dot blot,IHC |
| 基因名称 | COL2A1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
| 计算分子量 | 142kDa |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed,paraffin embedded tissues.This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross-reactivity to Type I,III and V collagens.Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at 4℃ prior to opening.This product is stable at 4℃ as an undiluted liquid.Dilute only prior to immediate use.For extended storage,mix with an equal volume of glycerol,aliquot contents and freeze at -20℃ or below.Avoid cycles of freezing and thawing.Expiration date is one (1) year from date of opening. |
| 背景资料 | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure.For these reasons,it is often extremely difficult to generate antibodies with specificities to collagens.The development of ＆type＊ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.Biorab extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation.This preparation results in a native conformation of the protein.Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.Ideal for investigators involved in Cell Biology,Signal Transduction and Stem Cell research. |
| Uniprot ID | P02458 |
| 基因名全称 | Collagen alpha-1(II) chain |
| 推荐稀释比 | ELISA:1:5,000 - 1:50,000 IHC:1:50 - 1:400 IF Microscopy:User Optimized IP:1:100 WB:1:1,000 - 1:10,000 |
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